Analyst MKII supports a range of fluorescence microscopic imaging
assays by generic protocols for data recording on typical microscopy
systems and specific analysis
protocols using pre-configured pipelines accessible from the main
Image Analyst MKII provides the complete and extended version of the
plasma and mitochondrial membrane potential measurement technique
developed by Akos Gerencser and colleagues (
17). This is supported by protocols for data acquisition, image processing pipelines
for measurement of fluorescence intensities in image data from a
variety of formats and the intuitive Calibration Wizard dialog
designed for easy and routine use for biologists with no biophysical
or mathematical expertise required.
Over a hundred functions can be automated by simple, drag-and-drop,
flowchart based pipelines. 75 built-in,
menu-accessible pipelines support many basic and advanced image
processing tasks and a variety of biological applications. Routine pipeline
usage is greatly simplified now by using pipeline parameters to control key
image processing functions within the pipeline. For basic use, the user
sees only the pipeline parameters, but not the individual functions.
However built-in pipelines can be opened and arbitrarily modified.
Fluorescence time-lapse analysis made easy
Image Analyst MKII is a specialized image analysis tool for
fluorescence time-lapse microscopy live-cell imaging in biomedical sciences.
The software was developed for speed and ease of use during routine work of the
biologist with no programming or scripting skills. Use this tool to extract graphs of
biological parameters from time-lapse image sequences, like ion concentrations, membrane potentials,
organelle shape changes and motion.