Fluorescence microscopy image analysis
automation - time series - physiology

Fluorescence time-lapse analysis made easy

   Image Analyst MKII is an image analysis application for fluorescence microscopy in biomedical sciences.

    Image Analyst MKII extracts graphs of biological parameters from time-lapse image sequences, like ion concentrations, membrane potentials, organelle shape changes and motion. It perfroms measurements in nuclei or cells with segmentation or ROIs in images from fixed specimens.

   It is ideal for processing recordings with complexity ranging from single frames to multi-dimensional data sets and organize technical and experimental replicates straight in Graphpad Prism or in Microsoft Excel.

Fluorescence microscopy and mitochndrial assays
Cell and mitochondrial
physiology assays

   Image Analyst MKII supports a range of fluorescence microscopic assays by generic protocols for data recording on typical microscopy systems and specific analysis protocols ranging from cell counting to ion concentrations, potentials and motility, using pre-configured pipelines accessible from interactive assay protocols or by menu selection.

Mitochondrial membrane potential assay
Mitochondrial membrane potential
measurment in absolute millivolts

   Turn fluorescence recordings of potentiometric probes straight into time courses of millivolt values. Image Analyst MKII provides the mitochondrial membrane potential measurement technology developed by Akos Gerencser and colleagues ( 17). This is supported by protocols for image acquisition, and an interactive protocol for image and data processing using the intuitive Membrane Potential Calibration Wizard dialog.

Interactive protocol
Interactive analysis protocols

   Image analysis cannot be simpler than using our interactive protocols. Beginners can learn quickly by clicking each step and watching the application performing the analysis. Advanced users can build elaborate analysis starting out with built-in pipelines.

Pipeline automation of fluorescence microscopy image analysis
Automate image analysis
with simple pipeline design

   Over a hundred functions can be automated by simple, drag-and-drop, flowchart based pipelines. 72 built-in, menu-accessible pipelines support many basic and advanced image processing tasks and a variety of biological applications. Routine pipeline usage is greatly simplified by using pipeline parameters to control key image processing functions within the pipeline. For basic use, the user sees only these key parameters, but all built-in pipelines can be edited and arbitrarily modified.

What`s New?

Image Analyst MKII - News

Release of version 4.0.0

  • Batch pipeline processor - automate with simple Excel tables
  • Pipeline Optimizer
  • MOV, MP4, H.264 import and export
  • and more...
Image Analyst MKII - News

Publication Alert

Natural Genetic Variation in Yeast Reveals That NEDD4 Is a Conserved Modifier of Mutant Polyglutamine Aggregation. G3 (Bethesda). 2018 Nov 6;8(11):3421-3431

  • Automated analysis of microplate imaging data set
  • Measurement of protein aggregation
Image Analyst MKII - News

International distributor

  • Image Analyst MKII is available worldwide through our international distributor, 2Checkout (formerly Avangate).
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