Fluorescence microscopy image analysis
automation - time series - physiology

Fluorescence microscopy and mitochndrial assays
Cell and mitochondrial
physiology assays

   Image Analyst MKII supports a range of fluorescence microscopic imaging assays by generic protocols for data recording on typical microscopy systems and specific analysis protocols using pre-configured pipelines accessible from the main menu.

Mitochondrial membrane potential assay
Mitochondrial membrane potential
measurment in absolute millivolts

   Image Analyst MKII provides the complete and extended version of the plasma and mitochondrial membrane potential measurement technique developed by Akos Gerencser and colleagues ( 17). This is supported by protocols for data acquisition, image processing pipelines for measurement of fluorescence intensities in image data from a variety of formats and the intuitive Calibration Wizard dialog designed for easy and routine use for biologists with no biophysical or mathematical expertise required.

Pipeline automation of fluorescence microscopy image analysis
Automate image analysis
with simple pipeline design

   Over a hundred functions can be automated by simple, drag-and-drop, flowchart based pipelines. 75 built-in, menu-accessible pipelines support many basic and advanced image processing tasks and a variety of biological applications. Routine pipeline usage is greatly simplified now by using pipeline parameters to control key image processing functions within the pipeline. For basic use, the user sees only the pipeline parameters, but not the individual functions. However built-in pipelines can be opened and arbitrarily modified.

Fluorescence time-lapse analysis made easy

   Image Analyst MKII is a specialized image analysis tool for fluorescence time-lapse microscopy live-cell imaging in biomedical sciences. The software was developed for speed and ease of use during routine work of the biologist with no programming or scripting skills. Use this tool to extract graphs of biological parameters from time-lapse image sequences, like ion concentrations, membrane potentials, organelle shape changes and motion.


What`s New?

Image Analyst MKII - News

Publication Alert

Suppressors of Superoxide-H2O2 Production at Site IQ of Mitochondrial Complex I Protect against Stem Cell Hyperplasia and Ischemia-Reperfusion Injury, Cell Metabolism [Epub ahead of print]

  • In situ succinate dehydrogenase activity assay with microscopic densitometry in multiwell plates
Image Analyst MKII - News

Publication Alert

Measurement of the Absolute Magnitude and Time Courses of Mitochondrial Membrane Potential in Primary and Clonal Pancreatic Beta-Cells, PLoS One. 2016;11(7):e0159199.

  • ΔψM and ΔψP in intact cells
  • Mitochondria:Cell volume fraction
  • Single-cell analysis
Image Analyst MKII - News

Publication Alert

Alterations in voltage-sensing of the mitochondrial permeability transition pore in ANT1-deficient cells, Sci Rep. 2016 May 25;6:26700

  • ΔψM and ΔψP in intact cells
  • Mitochondrial swelling with thinness ratio
Image Analyst MKII - News

Release of version 3.2.0

  • High DPI display support
  • Multi-dimensional TIFF folder open