Image Analyst MKII provides complex image processing tasks in a biologist-friendly manner.

Fluorescence microscopy image analysis
automation - time series - physiology

Release of version 4.0.0

  • Batch pipeline processor - automate with simple Excel tables
  • Pipeline Optimizer
  • MOV, MP4, H.264 import and export
  • and more...

Publication Alert

Natural Genetic Variation in Yeast Reveals That NEDD4 Is a Conserved Modifier of Mutant Polyglutamine Aggregation. G3 (Bethesda). 2018 Nov 6;8(11):3421-3431

  • Automated analysis of microplate imaging data set
  • Measurement of protein aggregation

International distributor

  • Image Analyst MKII is available worldwide through our international distributor, 2Checkout (formerly Avangate).

Feature highlight

Interactive protocol: Measure fluorescence intensity or ratio in time courses

  • A step-by step guide how to choose and set up an analysis pipeline

Feature highlight

Pipeline Optimizer: The Pipeline Optimizer finds optimal parameters of a pipeline that achieve a user defined goal.


Feature highlight

Batch Pipeline Processor: It uses an Excel table to define what image files to open and which pipelines with what parameters to run on them, and where to export results.

Release of version 3.5.5

  • New interactive protocols and pipelines
  • Improvements for smoother operation

Publication Alert

Metabolic activation-driven mitochondrial hyperpolarization predicts insulin secretion in human pancreatic beta-cells. Biochim Biophys Acta. 2018

  • Mitochondrial membrane potential and volume fraction assay
  • Normalization of respirometry data with cell counting

Release of version 3.5.3

  • About 20 improvements

Release of version 3.5.1

  • Going 64-bit
  • Organize technical and experimental replicates of time courses directly in Graphpad Prism files
  • Interactive Primer including analysis protocols
  • Loads of convenience features for easier and faster analysis

Publication Alert

ROS Control Mitochondrial Motility through p38 and the Motor Adaptor Miro/Trak. Cell Rep. 2017 Nov 7;21(6):1667-1680

  • Mitochondrial motility assaying with Optical Flow using Image Analyst MKII

Publication Alert

Suppressors of Superoxide-H2O2 Production at Site IQ of Mitochondrial Complex I Protect against Stem Cell Hyperplasia and Ischemia-Reperfusion Injury, Cell Metabolism 2016 Oct 11;24(4):582-592

  • In situ succinate dehydrogenase activity assay with microscopic densitometry in multiwell plates

Publication Alert

Measurement of the Absolute Magnitude and Time Courses of Mitochondrial Membrane Potential in Primary and Clonal Pancreatic Beta-Cells, PLoS One. 2016;11(7):e0159199.

  • ΔψM and ΔψP in intact cells
  • Mitochondria:Cell volume fraction
  • Single-cell analysis

Publication Alert

Alterations in voltage-sensing of the mitochondrial permeability transition pore in ANT1-deficient cells, Sci Rep. 2016 May 25;6:26700

  • ΔψM and ΔψP in intact cells
  • Mitochondrial swelling with thinness ratio

Release of version 3.2.0

  • High DPI display support
  • TIFF folder open

March Newsletter:

Mitochondrial Morphology & Dynamics

February Newsletter

NAD(P)H Autofluorescence Analysis

The new website is launched.

December Newsletter

Release of version 3.1.0

November Newsletter

October Newsletter