Fluorescence microscopy image analysis
automation - time series - physiology

Fluorescence time-lapse analysis made easy

   Image Analyst MKII is a specialized image analysis application for fluorescence time-lapse microscopy live-cell imaging in biomedical sciences. The software was developed for speed and ease of use during routine work of biologists.

     Use this tool to extract graphs of biological parameters from time-lapse image sequences, like ion concentrations, membrane potentials, organelle shape changes and motion.

   Process recordings with complexity ranging from single frames to multi-dimensional data sets and organize technical and experimental replicates straight in Graphpad Prism or in Microsoft Excel.

Fluorescence microscopy and mitochndrial assays
Cell and mitochondrial
physiology assays

   Image Analyst MKII supports a range of fluorescence microscopic assays by generic protocols for data recording on typical microscopy systems and specific analysis protocols ranging from cell counting to ion concentrations, potentials and motility, using pre-configured pipelines accessible from interactive assay protocols or by menu selection.

Interactive protocol
Interactive analysis protocols

   Image analysis cannot be simpler than using our interactive protocols. Beginners can learn quickly by clicking each step and watching the application performing the analysis. Advanced users can build elaborate analysis starting out with built-in pipelines.

Mitochondrial membrane potential assay
Mitochondrial membrane potential
measurment in absolute millivolts

   Turn fluorescence recordings of potentiometric probes straight into time courses of millivolt values. Image Analyst MKII provides the mitochondrial membrane potential measurement technology developed by Akos Gerencser and colleagues ( 17). This is supported by protocols for image acquisition, and an interactive protocol for image and data processing using the intuitive Membrane Potential Calibration Wizard dialog.

Pipeline automation of fluorescence microscopy image analysis
Automate image analysis
with simple pipeline design

   Over a hundred functions can be automated by simple, drag-and-drop, flowchart based pipelines. 72 built-in, menu-accessible pipelines support many basic and advanced image processing tasks and a variety of biological applications. Routine pipeline usage is greatly simplified by using pipeline parameters to control key image processing functions within the pipeline. For basic use, the user sees only these key parameters, but all built-in pipelines can be edited and arbitrarily modified.

What`s New?

Image Analyst MKII - News

Release of version 3.5.5

  • New interactive protocols and pipelines
  • Improvements for smoother operation
Image Analyst MKII - News

Publication Alert

Metabolic activation-driven mitochondrial hyperpolarization predicts insulin secretion in human pancreatic beta-cells. Biochim Biophys Acta. 2018 [Free access until 10/3/2018]

  • Mitochondrial membrane potential and volume fraction assay
  • Normalization of respirometry data with cell counting
Image Analyst MKII - News

Release of version 3.5.3

  • Going 64-bit
  • Organize technical and experimental replicates of time courses directly in Graphpad Prism files
  • Interactive Primer including analysis protocols
  • Loads of convenience features for easier and faster analysis
Image Analyst MKII - News

Publication Alert

ROS Control Mitochondrial Motility through p38 and the Motor Adaptor Miro/Trak. Cell Rep. 2017 Nov 7;21(6):1667-1680

  • Mitochondrial motility assaying with Optical Flow using Image Analyst MKII
Image Analyst MKII - News

Publication Alert

Suppressors of Superoxide-H2O2 Production at Site IQ of Mitochondrial Complex I Protect against Stem Cell Hyperplasia and Ischemia-Reperfusion Injury, Cell Metabolism 2016 Oct 11;24(4):582-592

  • In situ succinate dehydrogenase activity assay with microscopic densitometry in multiwell plates using Image Analyst MKII
Image Analyst MKII - News

Publication Alert

Measurement of the Absolute Magnitude and Time Courses of Mitochondrial Membrane Potential in Primary and Clonal Pancreatic Beta-Cells, PLoS One. 2016;11(7):e0159199.

  • ΔψM and ΔψP in intact cells
  • Mitochondria:Cell volume fraction
  • Single-cell analysis