Image Analyst MKII provides complex image processing tasks in a biologist-friendly manner.

Fluorescence microscopy image analysis
automation - time series - physiology

Fluorescence  Microscopy time-lapse analysis made easy

  Image Analyst MKII is a microscopy image analysis platform to visualize and measure biological parameters. Typical workflows include graphing time-lapse image data and high content analysis.  Image Analyst MKII is ideal for processing recordings with complexity ranging from single frames to multi-dimensional data sets.

  • Interactive time lapse analysis for ion concentrations and membrane potentials.
  • Automated microplate-based analysis for cytometry and high content analysis.
  • Pipeline-based analysis of native image formats without intermediate image data accumulated.
  • Biologically decoded presentation of analysis
  • Organize technical and experimental replicates straight in Graphpad Prism or in Microsoft Excel.

Interactive analysis protocols

   Image analysis cannot be simpler than using our interactive protocols. Biologists new to microscopic image analysis can get to routine quickly by clicking each step and watching the application performing the analysis. Ready-to-go interactive analysis protocols included for cell counting, cytometry, histometry, intensity and ratio time courses and more ...

Interactive protocol
Fluorescence microscopy and mitochndrial assays

Cell and mitochondrial
physiology and cytometry assays

   Image Analyst MKII supports a range of fluorescence and bright field microscopic assays by generic protocols for data recording on typical microscopy systems and specific analysis protocols ranging from cell counting to ion concentrations, potentials and motility, using pre-configured pipelines accessible from interactive assay protocols or by menu selection. Analysis pipelines are applicable to wide field (epi-) fluorescence, confocal and two-photon microscopy.

Mitochondrial membrane potential assay
Mitochondrial membrane potential
measurment in absolute millivolts

   Turn fluorescence recordings of potentiometric probes straight into time courses of millivolt values. Image Analyst MKII provides the mitochondrial membrane potential measurement technology developed by Akos Gerencser and colleagues ( 17). This is supported by protocols for image acquisition, and an interactive protocol for image and data processing using the intuitive Membrane Potential Calibration Wizard dialog.

Pipeline automation of fluorescence microscopy image analysis

Automate image analysis
with simple pipeline design

   Over a hundred functions can be automated by simple, drag-and-drop, flowchart based pipelines. 118 built-in, menu-accessible pipelines support many basic and advanced image processing tasks and a variety of biological applications. Routine pipeline usage is greatly simplified by using pipeline parameters to control key image processing functions within the pipeline. For basic use, the user sees only these key parameters, but all built-in pipelines can be edited and arbitrarily modified.

What`s New?

Image Analyst MKII - Publications
Cellular Senescence

A Fully-Automated Senescence Test (FAST) for the high-throughput quantification of senescence-associated markers
Image Analyst MKII takes a center place in our new analysis method just published on bioRxiv. We provide an automated workflow for image and data analysis, from microplates to graphing.

Image Analyst MKII - Release

Release of version 4.2.7

  • Improved Nikon *.nd2 handling
  • ROI tools
  • Improved z-stabilizer
  • and more...
Image Analyst MKII - Release

Image Analyst MKII Pipelines on GitHub

Image Analyst MKII - Release

Release of version 4.2.5

  • Bright field Color Deconvolution with PCA
  • AI Create Training Set
  • GitHub support
  • More ROI annotation handling
  • and more...
Image Analyst MKII - Release

Release of version 4.2.2

  • AI-based segmentation and ROI drawing using Cellpose
  • Improved Bio-Formats support, and pyramidal format handling
  • Use Frame Labels, ROI annotations and edit time points
  • Updated histometry/cytometry pipelines
  • and more...
Image Analyst MKII - Publications
Publication Alert

Integrating AI cell segmentation to find cells based on NADH autofluorescence
Site IQ in mitochondrial complex I generates S1QEL-sensitive superoxide/hydrogen peroxide in both the reverse and forward reactions. In Biochem J.

  • Automated ROI-based time lapse analysis using Cellpose
  • Pipeline integration for Elastix through command line execution
Image Analyst MKII - Publications
Publication Alert

The complete protocol for ΔψM, ΔψP and mito:cell volume fraction assays have been published:
Unbiased Millivolts Assay of Mitochondrial Membrane Potential in Intact Cells. In Methods in Molecular Biology

  • Sample and media preparation
  • Fluorescence microscopy recording
  • Image and data analysis
  • Troubleshooting