Fluorescence Microscopy time-lapse analysis made easy
Image Analyst MKII is an image analysis
platform for microscopy in biomedical sciences, with an emphasis on
time-lapse analysis and microplate automation.
Image Analyst MKII extracts
graphs of
biological parameters from time-lapse image sequences, like ion concentrations, membrane potentials,
organelle shape changes and motion in live specimens. It performs measurements in nuclei or cells with segmentation or ROIs in images from fixed specimens.
It is ideal for processing recordings with complexity ranging from single frames to multi-dimensional data sets and organize technical
and experimental replicates straight in
Graphpad Prism or in
Microsoft Excel.
Image analysis cannot be simpler than using our interactive protocols.
Biologists new to microscopic image analysis can get to routine quickly by clicking each step and watching the application performing the analysis.
Ready-to-go interactive analysis protocols included for cell counting,
cytometry, histometry, intensity and ratio time courses and more ...
Image Analyst MKII supports a range of
fluorescence and bright field microscopic assays by generic
protocols for data recording on typical microscopy
systems and specific analysis protocols ranging from
cell counting to ion concentrations, potentials and
motility, using
pre-configured pipelines accessible from interactive
assay protocols or by menu selection. Analysis pipelines are applicable to wide field (epi-) fluorescence, confocal and two-photon microscopy.
Turn fluorescence recordings of potentiometric probes straight into time courses of millivolt values. Image Analyst MKII provides the mitochondrial membrane potential measurement technology
developed by Akos Gerencser and colleagues (
17). This is supported by protocols for image acquisition, and an interactive protocol for image and data processing using the intuitive Membrane Potential Calibration Wizard dialog.
Over a hundred functions can be automated by simple, drag-and-drop,
flowchart based pipelines. 118 built-in,
menu-accessible pipelines support many basic and advanced image
processing tasks and a variety of biological applications. Routine pipeline
usage is greatly simplified by using pipeline parameters to control key
image processing functions within the pipeline. For basic use, the user
sees only these key parameters, but all built-in pipelines can be edited and arbitrarily modified.
The complete protocol for ΔψM, ΔψP and mito:cell volume fraction assays have been published:
Unbiased Millivolts Assay of
Mitochondrial Membrane Potential in Intact Cells. In Methods in Molecular Biology
Superoxide produced by mitochondrial site IQ inactivates cardiac succinate dehydrogenase and induces hepatic steatosis in Sod2 knockout mice. Free Radic Biol Med
. 2021 Feb 20;164:223-232
The pipeline optimize was used to create a sensitive quantification of fat droplets
Succinate dehydrogenase activity was analyzed using micro densitometry
