Protocols for assays using arbitrary/certain microscopy systems and image analysis in Image Analyst MKII
- Legacy mitochondrial membrane potential assay protocols - current protocols are published in Methods in Molecular Biology
- Reactive oxygen species assay protocols
- Assay of levels of reactive oxygen species using DHE ratioing
- Assay of levels of reactive oxygen species with normalized rate of DCFDA oxidation
- Assay of mitochondrial levels of reactive oxygen species using MitoSOX:MitoTracker Deep red end point measurement
- Assay of mitochondrial levels of reactive oxygen species using OH-ethidium rate measurement and MitoSOX:TMRM:MitoTracker Deep Red double normalization
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Mitochondrial dynamics assay protocols
- Measurements of morphological properties of mitochondria e.g. length or numbers using built-in pipelines
- Measurements of mitochondrial morphological properties, velocities and intensities in populations or individual mitochondria
- Mitochondrial swelling measurement with "ratiometric optimized spatial filtering technique" AKA "thinness ratio"
- Mitochondrial velocity assays using optical flow assay protocols
- Organelle motion assay with Optical Flow
- Image acquisition for mitochondrial motion in Elements and analysis in Image Analyst MKII
- Image acquisition for mitochondrial motion in Metamorph and analysis in Image Analyst MKII
- Image acquisition for mitochondrial motion in Zeiss LSM and analysis in Image Analyst MKII
- Working with Optical Flow images
- Fine Tuning Optical Flow
- Measurement of velocities and fluorescence intensities over single mitochondria
- Metabolism assay protocols
- Other