Overview
The aim of the development of Image Analyst MKII was to create a speed optimized and user friendly image analysis environment primarily for the field of cell and mitochondrial physiology. Image Analyst MKII provides functionality for unbiased image analysis and convenient processing of multi-position and microplate-based image sets by streamlined but flexible analysis automation.
As input, the Image Analyst MKII reads single or multi-channel X,Y,t or X,Y,Z,t image sequences. For this speed optimized internal readers or interfacing with Bio-Formats reader library are available. You can perform math with fluorescence intensities using floating point precision and speed offered by multi-core CPUs. The image processing functions and complex pipelines provided by the Image Analyst MKII has been proved to be useful for biological, time lapse image processing, and validated in peer-reviewed publications.
The output of Image Analyst MKII is the extracted biological information as a function of time (or end points) in tabular format; in text files or directly transferred to Microsoft Excel or Graphpad Prism files. Microplate data can be outputted in plate format. Organize multi-position recordings by assigning condition names and automatically pooling technical replicates and then collecting data from multiple experiments in Graphpad Prism files. Processed images can be exported as RGB pictures or movies.
Features
Key features of Image Analyst MKII:
- Explore multi-dimensional time-lapse data
- Pipeline automation of end-point and time series image analysis
- Control Image Analyst MKII from interactive protocols to speed up routine work or to educate novice users
- Optimized for 2D time-lapse analysis, and analysis of 3D time lapses as projection images
- Easy handling of multiwell plate data with plate-formatted Excel output
- Intensity, morphology, counting and motility/tracking assays
- End point analysis for cell counting such as cell death or viability assays, optical density measurements for histochemical or enzyme activity stains like sa-bgal or formazan
- Pool data of technical replicate positions and calculate statistics on experimental replicates by resampling time courses and organizing in Graphpad Prism
- Cellular and mitochondrial physiology and bioenergetics-specific assays with protocols
- Direct reading of microscopy and common image and video file formats without conversion
- Precise reading of time stamps in lsm, nd2, nd, tif and stk files
- Export of processed, RGB images and movies to tif, jpg, mov, avi, mp4, including mpeg and h.264 compressions
- Intuitive pipeline building without programming skills
- Fast prototyping of analysis - the parameter bar allows instant access to adjustments in processing
- Pipeline optimizer using differential evolution to find optimal set of parameters
- Platform-specific optimized code for computation speed
- Fast floating point precision image processing
- High speed parallel computing - utilize your multi-core CPU
- 64-bit memory access to handle large recordings