Fluorescence microscopy image analysis
automation - time series - physiology

Overview

    The aim of the development of Image Analyst MKII was to create a speed optimized and user friendly image analysis environment primarily for the field of cell and mitochondrial physiology. Image Analyst MKII provides functionality for unbiased image analysis and convenient processing of multi-position and microplate-based image sets by streamlined but flexible analysis automation.

    As input, the Image Analyst MKII reads single or multi-channel X,Y,t or X,Y,Z,t image sequences. For this speed optimized internal readers or interfacing with Bio-Formats reader library are available.  You can perform math with fluorescence intensities using floating point precision and speed offered by multi-core CPUs. The image processing functions and complex pipelines provided by the Image Analyst MKII has been proved to be useful for biological, time lapse image processing, and validated in peer-reviewed publications.

    The output of Image Analyst MKII is the extracted biological information as a function of time in tabular format; in text files or directly transferred to Excel. Microplate data can be outputted in plate format. Processed images can be exported as RGB pictures or movies.  

Features

Key features of Image Analyst MKII:

  • Explore your multi-dimensional time-lapse data
  • Pipeline automation of end-point and time series image analysis
  • Optimized for 2D time-lapse analysis, and analysis of 3D time lapses as projection images
  • Easy handling of multiwell plate data with plate-formatted Excel output
  • Cellular and mitochondrial physiology and bioenergetics-specific assays with protocols
  • Direct reading of microscopy file formats without conversion
  • Precise reading of time stamps in lsm, nd2, nd, tif and stk files
  • Easy pipeline building without programming skills
  • Fast prototyping of analysis - the parameter bar allows instant access to adjustments in processing
  • Platform-specific optimized code for computation speed
  • Fast floating point precision image processing
  • High speed parallel computing - utilize your multi-core CPU