Absolute and Unbiased Mitochondrial Membrane Potential Assay

   Commonly used mitochondrial membrane potential (ΔψM) assays often lead to data misinterpretation. This is because all of the used probes are influenced by multiple properties of cells other than ΔψM.

  Our technology is based on biophysical modeling of ΔψM probe behavior to back-calculate potentials from time courses of fluorescence intensities. This enables absolute millivolts readout, and an unbiased comparison of different samples, accounting for geometric, binding and ΔψP effects.

The Theory

How absolute millivolts are calculated

See how effects of ΔψP, cell and mitochondrial geometry and fluorescence background accounted for to calculate mV values.

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In Practice

The Membrane Potential Calibration Wizard

This interactive dialog works hand in hand with the provided protocol to calibrate potentials without theoretical knowledge.

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Try it out

• Download Image Analyst MKII
• Download example image data
• Follow interactive protocol
• Video tutorial
• Read in depth protocol

See the technology in publications

• Published in-depth protocol (61)
• In human stem cells (14)
• In rat neurons (17)
• In human and rodent β-cells (26,31,40)
• In osteoblasts (40)
• In MCF7 (49)
• In hepatocellular carcinoma (51)
• In H29C, BJ1, HepG2 cells, primary cardiomyocytes (61)

Mitochondrial Membrane Potential Assay Protocol

Protocol

Protocol explaining experimental design, microscopy setup and image analysis

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