Mitochondria:cell volume fraction measurement in INS-1E insulinoma cells. Red, mitochondria (MitoTracker Red); Green, cells (calcein-AM). From: (17).
Mitochondria:cell volume fraction measurement in INS-1E insulinoma cells. Binarized image. Yellow, mitochondria (MitoTracker Red); Green, cells (calcein-AM). From: (17).
Mitochondria:cell volume fraction measurement in INS-1E insulinoma cells. Motage of Raw fluorecence (top left), Binarized calcein fluorescence (top right), highpass filtered MitoTracker Red (bottom left) and Calcein fluorescence (gray) overlaid by mitochondrial segments (bottom right). From: (17).
Mitochondria:cell volume fraction measurement in rat cortical neurons. Highpass filtered MitoTracker Red (left) and Calcein fluorescence (gray) overlaid by mitochondrial segments (right). From: (17).
Mitochondrial volume fractionator in human dopaminergic neurons differentiated from induced pluripotent stem cells.
Different phases of image processing for measurement of mitochondria:cell volume fraction.
From: (24).
Mitochondrial volume fractionator in human dopaminergic neurons differentiated from induced pluripotent stem cells.
Different phases of image processing for measurement of mitochondria:cell volume fraction.
From: (24).
Montage of confocal micrographs of human dispersed islet cells represented as a composite of artificially colored fluorescence channels and processed images used to calculate mitochondria:cell volume fractions in live β-cells. Three imaging modalities of the same individual cells are overlaid by image registration; 1) live imaging: red, mitochondria (MitoTracker Red); blue, cells (calcein-AM); 2) immunostained, fixed-specimen imaging: green: insulin immunofluorescence; white, nuclei (Hoechst 33342); 3) image processing: various colored shapes indicate mitochondrial profiles. One tile of the montage is 22.5 µm wide. From: (26).
Confocal micrograph of human dispersed islet cells represented as a composite of artificially colored fluorescence channels and processed images used to calculate mitochondria:cell volume fractions in live β-cells. Three imaging modalities of the same individual cells are overlaid by image registration; 1) live imaging: red, mitochondria (MitoTracker Red); blue, cells (calcein-AM); 2) immunostained, fixed-specimen imaging: green: insulin immunofluorescence; white, nuclei (Hoechst 33342); 3) image processing: various colored shapes indicate mitochondrial profiles. The image 22.5 µm wide. From: (26).