An overnight recording on Hoechst 33342-stained hESCs was processed with segmentation and tracking in Image Analyst MKII. The cell divisions were automatically annotated with the Special/Count Cell Division and Cell Death function in the tracked time series. Related to (14).
A mammalian cell culture expressing a nuclear red fluorescent protein and a cytosolic yellow cameleon calcium sensor was overnight imaged and processed with segmentation and tracking in Image Analyst MKII. Nuclei were then colored uniformly to the measured calcium ratio using the Measure Object Intensity function.
Human fibroblasts were stained with Hoechst 33342 and phalloidin-Alexa Fluor 647 and segmented using the nuclear stain as seed. Solid colors indicate different segments (cells).
Human fibroblasts stained with Hoechst 33342 were overnight imaged and processed with segmentation and tracking in Image Analyst MKII.
Live human dispersed pancreatic islet cell cultures were stained with Hoechst 33342, MitoSOX and MitoTracker Deep Red, and after fixation for insulin immunofluorescence. Image segmentation was performed to selectively revel MitoTracker and insulin positive cells, seeded by the nuclear stain. This was used to determine MitoSOX:MitoTracker Deep Red ratio selectively in pancreatic β-cells in (26).
Protein aggregates revealed in Huntingtin-GFP expressing human cells using a combination of high pass filtering and segmentation classifiers in Image Analyst MKII.
Post-hoc staining with DRAQ5 was used to guide segmentation of Fura-2 images. From: (16)
Post-hoc staining with Hoechst 33342 was used to guide segmentation of Fura-red images. From: (16)
Post-hoc staining with DRAQ5 was used to guide segmentation of FLIPR membrane potential probe (aka. PMPI) images. From: (16)